非转移性黑色素瘤糖蛋白B对小鼠黑色素细胞色素生成的影响

doi:10.11843/j.issn.0366-6964.2017.09.010

Abstract

Abstract:

This study aims to investigate whether GPNMB differentially expresses in the skin of mice with various hair colors and to determine its effect on the melanin synthesis. Three approximately 12-day-old healthy C57BL/6 mice were randomly selected from strains with black, brown and gray coat colors, respectively. Three pieces of dorsal skin tissue were collected from each mouse using a skin collector after shaving. Immunohistochemistry assay revealed that GPNMB was mainly located in the hair follicle matrix, the inner and outer root sheath, and the dermal papilla in the skin with various hair colors. The Western blot analysis and qRT-PCR assay showed that the expression level of GPNMB was the lowest in the skin of gray mice. Compared with the control group, relative levels of MC1R, β-catenin and SLC45A2 significantly increased in mouse melanocytes overexpressing GPNMB.we detected the levels of GPNMB and MC1R in mouse melanocytes which had been radiated by UVB. The results showed that UVB had an impact on the GPNMB and MC1R expression, and the level of GPNMB was various with different UVB radiation doses.The above results suggested that GPNMB was differentially expressed in the skin of mice with different hair colors, and it was involved in the formation of the hair color by regulating the melanin synthesis.

CLC Number:

Q511

ZHAO Bing-ling, CHEN Tian-zhi, YANG Yu-jing, YU Xiu-ju, YANG Lin-pei, DONG Chang-sheng. Effects of GPNMB on Melanogenesis in Mouse Melanocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1654-1664.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.09.010

https://www.xmsyxb.com/EN/Y2017/V48/I9/1654

References

[1] CICHOREK M, WACHULSKA M, STASIEWICZ A, et al. Skin melanocytes:biology and development[J]. Postepy Dermatol Alergol, 2013, 30(1):30-41.
[2] CHOI W, MIYAMURA Y, WOLBER R, et al. Regulation of human skin pigmentation in situ by repetitive UV exposure:molecular characterization of responses to UVA and/or UVB[J]. J Invest Dermatol, 2010, 130(6):1685-1696.
[3] MIYAMURA Y, COELHO S G, SCHLENZ K, et al. The deceptive nature of UVA tanning versus the modest protective effects of UVB tanning on human skin[J]. Pigment Cell Melanoma Res, 2011, 24(1):136-147.
[4] YUN C Y, YOU S T, KIM J H, et al. p21-activated kinase 4 critically regulates melanogenesis via activation of the CREB/MITF and β-catenin/MITF pathways[J]. J Invest Dermatol, 2015, 135(5):1385-1394.
[5] LEVY C, KHALED M, FISHER D E. MITF:master regulator of melanocyte development and melanoma oncogene[J]. Trends Mol Med, 2006, 12(9):406-414.
[6] STEINGRIMSSON E, COPELAND N G, JENKINS N A. Melanocytes and the microphthalmia transcription factor network[J]. Annu Rev Genet, 2004, 38:365-411.
[7] HERSHEY C L, FISHER D E. Genomic analysis of the Microphthalmia locus and identification of the MITF-J/Mitf-J isoform[J]. Gene, 2005, 347(1):73-82.
[8] FUSE N, YASUMOTO K I, SUZUKI H, et al. Identification of a melanocyte-type promoter of the microphthalmia-associated transcription factor gene[J]. Biochem Biophys Res Commun, 1996, 219(3):702-707.
[9] SCHMIDT C, PATEL K. Wnts and the neural crest[J]. Anat Embryol Berl, 2005, 209(5):349-355.
[10] YASUMOTO K I, YOKOYAMA K, TAKAHASHI K, et al. Functional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosinase family genes[J]. J Biol Chem, 1997, 272(1):503-509.
[11] KIM D S, HWANG E S, LEE J E, et al. Sphingosine-1-phosphate decreases melanin synthesis via sustained ERK activation and subsequent MITF degradation[J]. J Cell Sci, 2003, 116(Pt 9):1699-1706.
[12] HAYASHI E, HACHIYA K, KOJO S, et al. α-MSH stimulation contributes to TGF-β1 production via MC1R-MITF signaling pathway in melanoma cell[J]. Inflammat Regenerat, 2015, 35(5):244-254.
[13] WETERMAN M A J, AJUBI N, VAN DINTER I M R, et al. nmb, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts[J]. Int J Cancer, 1995, 60(1):73-81.
[14] DU J Y, FISHER D E. Identification of Aim-1 as the underwhite mouse mutant and its transcriptional regulation by MITF[J]. J Biol Chem, 2002, 277(1):402-406.
[15] VITAVSKA O, WIECZOREK H. The SLC45 gene family of putative sugar transporters[J]. Mol Aspects Med, 2013, 34(2-3):655-660.
[16] GUTKNECHT M, GEIGER J, JOAS S, et al. The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells[J]. Cell Commun Signal, 2016, 13:19.
[17] ZHANG P, LIU W, ZHU C S, et al. Silencing of GPNMB by siRNA inhibits the formation of melanosomes in melanocytes in a MITF-independent fashion[J]. PLoS One, 2012, 7(8):e42955.
[18] THEOS A C, WATT B, HARPER D C, et al. The PKD domain distinguishes the trafficking and amyloidogenic properties of the pigment cell protein PMEL and its homologue GPNMB[J]. Pigment Cell Melanoma Res, 2013, 26(4):470-486.
[19] TOMIHARI M, HWANG S H, CHUNG J S, et al. Gpnmb is a melanosome-associated glycoprotein that contributes to melanocyte/keratinocyte adhesion in a RGD-dependent fashion[J]. Exp Dermatol, 2009, 18(7):586-595.
[20] CHEN T Z, WANG H D, LIU Y, et al. Ocular Albinism Type 1 regulates melanogenesis in mouse melanocytes[J]. Int J Mol Sci, 2016, 17(10):1596.
[21] WU B, SONDAG G R, MALCUIT C, et al. Macrophage-associated osteoactivin/GPNMB mediates mesenchymal stem cell survival, proliferation, and migration via a CD44-dependent mechanism[J]. J Cell Biochem, 2016, 117(7):1511-1521.
[22] ONO Y, TSURUMA K, TAKATA M, et al. Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na⁺/K⁺-ATPase[J]. Sci Rep, 2016, 6:23241.
[23] COSTIN G E, VALENCIA J C, VIEIRA W D, et al. Tyrosinase processing and intracellular trafficking is disrupted in mouse primary melanocytes carrying the underwhite (uw) mutation. A model for oculocutaneous albinism (OCA) type 4[J]. J Cell Sci, 2003, 116(pt15):3203-3212.
[24] CULLINANE A R, VILBOUX T, O'BRIEN K, et al. Homozygosity mapping and whole-exome sequencing to detect SLC45A2 and G6PC3 mutations in a single patient with oculocutaneous albinism and neutropenia[J]. J Invest Dermatol, 2011, 131(10):2017-2025.
[25] HEARING V J. Biogenesis of pigment granules:a sensitive way to regulate melanocyte function[J]. J Dermatol Sci, 2005, 37(1):3-14.
[26] ANDERSON M G, SMITH R S, HAWES N L, et al. Mutations in genes encoding melanosomal proteins cause pigmentary glaucoma in DBA/2J mice[J]. Nat Genet, 2002, 30(1):81-85.
[27] LOFTUS S K, ANTONELLIS A, MATERA I, et al. Gpnmb is a melanoblast-expressed, MITF-dependent gene[J]. Pigment Cell Melanoma Res, 2009, 22(1):99-110.
[28] SCHALLREUTER K U, KOTHARI S, CHAVAN B, et al. Regulation of melanogenesis-controversies and new concepts[J]. Exp Dermatol, 2008, 17(5):395-404.
[29] HAASS N K, SMALLEY K S M, LI L, et al. Adhesion, migration and communication in melanocytes and melanoma[J]. Pigment Cell Res, 2005, 18(3):150-159.
[30] 赵兵令, 王海东, 陈天直, 等. 小鼠毛囊不同生长周期中MITF下游色素相关基因的定位表达及相关性分析[J]. 中国生物化学与分子生物学报, 2017, 33(2):198-206.
ZHAO B L, WANG H D, CHEN T Z, et al. Localization and correlation analysis of MITF downstream pigmentation-related genes at different growth cycle of mouse hair follicle[J]. Chinese Journal of Biochemistry and Molecular Biology, 2017, 33(2):198-206. (in Chinese)
[31] SCHEPSKY A, BRUSER K, GUNNARSSON G J, et al. The microphthalmia-associated transcription factor mitf interacts with β-catenin to determine target gene expression[J]. Mol Cell Biol, 2006, 26(23):8914-8927.
[32] CHELI Y, LUCIANI F, KHALED M, et al. αMSH and cyclic AMP elevating agents control melanosome pH through a protein kinase A-independent mechanism[J]. J Biol Chem, 2009, 284(28):18699-18706.
[33] WALKER W P, GUNN T M. Shades of meaning:the pigment-type switching system as a tool for discovery[J]. Pigment Cell Melanoma Res, 2010, 23(4):485-495.
[34] BUSCÀ R, ABBE P, MANTOUX F, et al. Ras mediates the cAMP-dependent activation of extracellular signal-regulated kinases (ERKs) in melanocytes[J]. EMBO J, 2000, 19(12):2900-2910.
[35] XU W D, GONG L M, HADDAD M M, et al. Regulation of microphthalmia-associated transcription factor MITF protein levels by association with the ubiquitin-conjugating enzyme hUBC9[J]. Exp Cell Res, 2000, 255(2):135-143.
[36] NAKANO Y, SUZUKI Y, TAKAGI T, et al. Glycoprotein nonmetastatic melanoma protein B (GPNMB) as a novel neuroprotective factor in cerebral ischemia-reperfusion injury[J]. Neuroscience, 2014, 277:123-131.
[37] BENTLEY N J, EISEN T, GODING C R. Melanocyte-specific expression of the human tyrosinase promoter:activation by the microphthalmia gene product and role of the initiator[J]. Mol Cell Biol, 1994, 14(12):7996-8006.
[38] 赵兵令, 李亚楠, 陈天直, 等. GPNMB通过调控MITF下游色素相关基因表达影响黑色素细胞色素的生成[J]. 中国农业科学, 2017, 50(7):1334-1342.
ZHAO B L, LI Y N, CHEN T Z, et al. GPNMB affects melanin synthesis in the melanocytes via MITF to regulate the downstream pigmental genes[J]. Scientia Agricultura Sinica, 2017, 50(7):1334-1342. (in Chinese)
[39] SATO S, ROBERTS K, GAMBINO G, et al. CBP/p300 as a co-factor for the Microphthalmia transcription factor[J]. Oncogene, 1997, 14(25):3083-3092.
[40] VACHTENHEIM J, BOROVANSKY J. "Transcription physiology" of pigment formation in melanocytes:central role of MITF[J]. Exp Dermatol, 2010, 19(7):617-627.
[41] ANGHEL S I, CORREA-ROCHA R, BUDINSKA E, et al. Breast cancer suppressor candidate-1(BCSC-1) is a melanoma tumor suppressor that down regulates MITF[J]. Pigment Cell Melanoma Res, 2012, 25(4):482-487.
[42] PIERRAT M J, MARSAUD V, MAUVIEL A, et al. Expression of microphthalmia-associated transcription factor (MITF), which is critical for melanoma progression, is inhibited by both transcription factor GLI2 and transforming growth factor-β[J]. J Biol Chem, 2012, 287(22):17996-18004.
[43] LUCOTTE G, MERCIER G, DIéTERLEN F, et al. A decreasing gradient of 374F allele frequencies in the skin pigmentation gene SLC45A2, from the north of West Europe to North Africa[J]. Biochem Genet, 2010, 48(1-2):26-33.
[44] DOOLEY C M, SCHWARZ H, MUELLER K P, et al. Slc45a2 and V-ATPase are regulators of melanosomal pH homeostasis in zebrafish, providing a mechanism for human pigment evolution and disease[J]. Pigment Cell Melanoma Res, 2013, 26(2):205-217.
[45] BIN B H, BHIN J, YANG S H, et al. Membrane-associated transporter protein (MATP) regulates melanosomal pH and influences tyrosinase activity[J]. PLoS One, 2015, 10(6):e0129273.
[46] CUI R T, WIDLUND H R, FEIGE E, et al. Central role of p53 in the suntan response and pathologic hyperpigmentation[J]. Cell, 2007, 128(5):853-864.
[47] TADOKORO T, YAMAGUCHI Y, BATZER J, et al. Mechanisms of skin tanning in different racial/ethnic groups in response to ultraviolet radiation[J]. J Invest Dermatol, 2005, 124(6):1326-1332.

Effects of GPNMB on Melanogenesis in Mouse Melanocytes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments